Cross-linking Immunoprecipitation:Article Title: The Molecular Biology of FMRP: New Insights into Fragile X Syndrome
Article Snippet: .. Van Driesche 2019 ( 35 ) mouse cerebellar Purkinje cells (Pcp2-Cre) and granule cells (NeuroD1-Cre), Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 135 high confidence FMRP CLIP targets in Purkinje cells; 259 in granule cells, they are distinct Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Synaptic functions; microtubule, Cell signaling, GTPase signaling in Purkinje cells, GABAergic transmission in cerebellar granule cells Sawicka 2019 ( 36 ) mouse CA1 neurons (Camk2a-Cre) Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 327; different from cb granule neurons Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Signaling, transcription, synaptic development and function, microtubule organization, axon transport, autism, circadian rhythm Ascano 2012 ( 42 ) Human HEK293 cell lines Transfected HA-tagged FMRP, anti-HA ab (MMS-101P, Convance), PAR-CLIP >6000 coding sequence and 3’UTR ACUK, WGGA many Tran 2019 ( 43 ) Human postmortem adult human frontal cortex Anti-FMRP pAb (MBL, RN016P), CLIP-seq 4895 binds to A-to-I edited site; predominantly 3’UTR ACUG Synaptic functions, (focus was editing in autism, not FMRP targets) Li 2020 ( 44 ) Human human iPSC differentiated dorsal and ventral forebrain NPCs and neurons (four cell types) FLAG tag knocked into human FMR1 locus, FMRP-FLAG, anti-FLAG ab (Sigma Aldrich F1804), CLIP-seq 1653 total (1232 in dNPCs, 1234 in vNPCs, 629 in dNeurons, 721 in vNeurons coding sequences, long genes CUAC; UGGA; GAUG, AGGU, CAGC; unclear about G4 Neurogenesis, axon and dendrite morphogenesis, cytoskeleton, microtubule, RNA transport, histone modification (especially in NPCs), autism. ..
Transmission Assay:Article Title: The Molecular Biology of FMRP: New Insights into Fragile X Syndrome
Article Snippet: .. Van Driesche 2019 ( 35 ) mouse cerebellar Purkinje cells (Pcp2-Cre) and granule cells (NeuroD1-Cre), Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 135 high confidence FMRP CLIP targets in Purkinje cells; 259 in granule cells, they are distinct Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Synaptic functions; microtubule, Cell signaling, GTPase signaling in Purkinje cells, GABAergic transmission in cerebellar granule cells Sawicka 2019 ( 36 ) mouse CA1 neurons (Camk2a-Cre) Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 327; different from cb granule neurons Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Signaling, transcription, synaptic development and function, microtubule organization, axon transport, autism, circadian rhythm Ascano 2012 ( 42 ) Human HEK293 cell lines Transfected HA-tagged FMRP, anti-HA ab (MMS-101P, Convance), PAR-CLIP >6000 coding sequence and 3’UTR ACUK, WGGA many Tran 2019 ( 43 ) Human postmortem adult human frontal cortex Anti-FMRP pAb (MBL, RN016P), CLIP-seq 4895 binds to A-to-I edited site; predominantly 3’UTR ACUG Synaptic functions, (focus was editing in autism, not FMRP targets) Li 2020 ( 44 ) Human human iPSC differentiated dorsal and ventral forebrain NPCs and neurons (four cell types) FLAG tag knocked into human FMR1 locus, FMRP-FLAG, anti-FLAG ab (Sigma Aldrich F1804), CLIP-seq 1653 total (1232 in dNPCs, 1234 in vNPCs, 629 in dNeurons, 721 in vNeurons coding sequences, long genes CUAC; UGGA; GAUG, AGGU, CAGC; unclear about G4 Neurogenesis, axon and dendrite morphogenesis, cytoskeleton, microtubule, RNA transport, histone modification (especially in NPCs), autism. ..
Transfection:Article Title: The Molecular Biology of FMRP: New Insights into Fragile X Syndrome
Article Snippet: .. Van Driesche 2019 ( 35 ) mouse cerebellar Purkinje cells (Pcp2-Cre) and granule cells (NeuroD1-Cre), Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 135 high confidence FMRP CLIP targets in Purkinje cells; 259 in granule cells, they are distinct Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Synaptic functions; microtubule, Cell signaling, GTPase signaling in Purkinje cells, GABAergic transmission in cerebellar granule cells Sawicka 2019 ( 36 ) mouse CA1 neurons (Camk2a-Cre) Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 327; different from cb granule neurons Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Signaling, transcription, synaptic development and function, microtubule organization, axon transport, autism, circadian rhythm Ascano 2012 ( 42 ) Human HEK293 cell lines Transfected HA-tagged FMRP, anti-HA ab (MMS-101P, Convance), PAR-CLIP >6000 coding sequence and 3’UTR ACUK, WGGA many Tran 2019 ( 43 ) Human postmortem adult human frontal cortex Anti-FMRP pAb (MBL, RN016P), CLIP-seq 4895 binds to A-to-I edited site; predominantly 3’UTR ACUG Synaptic functions, (focus was editing in autism, not FMRP targets) Li 2020 ( 44 ) Human human iPSC differentiated dorsal and ventral forebrain NPCs and neurons (four cell types) FLAG tag knocked into human FMR1 locus, FMRP-FLAG, anti-FLAG ab (Sigma Aldrich F1804), CLIP-seq 1653 total (1232 in dNPCs, 1234 in vNPCs, 629 in dNeurons, 721 in vNeurons coding sequences, long genes CUAC; UGGA; GAUG, AGGU, CAGC; unclear about G4 Neurogenesis, axon and dendrite morphogenesis, cytoskeleton, microtubule, RNA transport, histone modification (especially in NPCs), autism. ..
Sequencing:Article Title: The Molecular Biology of FMRP: New Insights into Fragile X Syndrome
Article Snippet: .. Van Driesche 2019 ( 35 ) mouse cerebellar Purkinje cells (Pcp2-Cre) and granule cells (NeuroD1-Cre), Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 135 high confidence FMRP CLIP targets in Purkinje cells; 259 in granule cells, they are distinct Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Synaptic functions; microtubule, Cell signaling, GTPase signaling in Purkinje cells, GABAergic transmission in cerebellar granule cells Sawicka 2019 ( 36 ) mouse CA1 neurons (Camk2a-Cre) Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 327; different from cb granule neurons Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Signaling, transcription, synaptic development and function, microtubule organization, axon transport, autism, circadian rhythm Ascano 2012 ( 42 ) Human HEK293 cell lines Transfected HA-tagged FMRP, anti-HA ab (MMS-101P, Convance), PAR-CLIP >6000 coding sequence and 3’UTR ACUK, WGGA many Tran 2019 ( 43 ) Human postmortem adult human frontal cortex Anti-FMRP pAb (MBL, RN016P), CLIP-seq 4895 binds to A-to-I edited site; predominantly 3’UTR ACUG Synaptic functions, (focus was editing in autism, not FMRP targets) Li 2020 ( 44 ) Human human iPSC differentiated dorsal and ventral forebrain NPCs and neurons (four cell types) FLAG tag knocked into human FMR1 locus, FMRP-FLAG, anti-FLAG ab (Sigma Aldrich F1804), CLIP-seq 1653 total (1232 in dNPCs, 1234 in vNPCs, 629 in dNeurons, 721 in vNeurons coding sequences, long genes CUAC; UGGA; GAUG, AGGU, CAGC; unclear about G4 Neurogenesis, axon and dendrite morphogenesis, cytoskeleton, microtubule, RNA transport, histone modification (especially in NPCs), autism. ..
FLAG-tag:Article Title: The Molecular Biology of FMRP: New Insights into Fragile X Syndrome
Article Snippet: .. Van Driesche 2019 ( 35 ) mouse cerebellar Purkinje cells (Pcp2-Cre) and granule cells (NeuroD1-Cre), Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 135 high confidence FMRP CLIP targets in Purkinje cells; 259 in granule cells, they are distinct Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Synaptic functions; microtubule, Cell signaling, GTPase signaling in Purkinje cells, GABAergic transmission in cerebellar granule cells Sawicka 2019 ( 36 ) mouse CA1 neurons (Camk2a-Cre) Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 327; different from cb granule neurons Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Signaling, transcription, synaptic development and function, microtubule organization, axon transport, autism, circadian rhythm Ascano 2012 ( 42 ) Human HEK293 cell lines Transfected HA-tagged FMRP, anti-HA ab (MMS-101P, Convance), PAR-CLIP >6000 coding sequence and 3’UTR ACUK, WGGA many Tran 2019 ( 43 ) Human postmortem adult human frontal cortex Anti-FMRP pAb (MBL, RN016P), CLIP-seq 4895 binds to A-to-I edited site; predominantly 3’UTR ACUG Synaptic functions, (focus was editing in autism, not FMRP targets) Li 2020 ( 44 ) Human human iPSC differentiated dorsal and ventral forebrain NPCs and neurons (four cell types) FLAG tag knocked into human FMR1 locus, FMRP-FLAG, anti-FLAG ab (Sigma Aldrich F1804), CLIP-seq 1653 total (1232 in dNPCs, 1234 in vNPCs, 629 in dNeurons, 721 in vNeurons coding sequences, long genes CUAC; UGGA; GAUG, AGGU, CAGC; unclear about G4 Neurogenesis, axon and dendrite morphogenesis, cytoskeleton, microtubule, RNA transport, histone modification (especially in NPCs), autism. ..
Modification:Article Title: The Molecular Biology of FMRP: New Insights into Fragile X Syndrome
Article Snippet: .. Van Driesche 2019 ( 35 ) mouse cerebellar Purkinje cells (Pcp2-Cre) and granule cells (NeuroD1-Cre), Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 135 high confidence FMRP CLIP targets in Purkinje cells; 259 in granule cells, they are distinct Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Synaptic functions; microtubule, Cell signaling, GTPase signaling in Purkinje cells, GABAergic transmission in cerebellar granule cells Sawicka 2019 ( 36 ) mouse CA1 neurons (Camk2a-Cre) Anti-GFP mAbs (HtzGFP19C8 and HtzGFP19F7, Heiman 2008 ( 91 ); from FMRP-cTag mice 327; different from cb granule neurons Long, coding sequences none has ranked targets based on TRAP-seq of same cell types; Signaling, transcription, synaptic development and function, microtubule organization, axon transport, autism, circadian rhythm Ascano 2012 ( 42 ) Human HEK293 cell lines Transfected HA-tagged FMRP, anti-HA ab (MMS-101P, Convance), PAR-CLIP >6000 coding sequence and 3’UTR ACUK, WGGA many Tran 2019 ( 43 ) Human postmortem adult human frontal cortex Anti-FMRP pAb (MBL, RN016P), CLIP-seq 4895 binds to A-to-I edited site; predominantly 3’UTR ACUG Synaptic functions, (focus was editing in autism, not FMRP targets) Li 2020 ( 44 ) Human human iPSC differentiated dorsal and ventral forebrain NPCs and neurons (four cell types) FLAG tag knocked into human FMR1 locus, FMRP-FLAG, anti-FLAG ab (Sigma Aldrich F1804), CLIP-seq 1653 total (1232 in dNPCs, 1234 in vNPCs, 629 in dNeurons, 721 in vNeurons coding sequences, long genes CUAC; UGGA; GAUG, AGGU, CAGC; unclear about G4 Neurogenesis, axon and dendrite morphogenesis, cytoskeleton, microtubule, RNA transport, histone modification (especially in NPCs), autism. ..
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